Article / The World Journal of MEN’s HEALTH
Therapeutic Effects of Mesenchymal Stem Cell Conditioned Medium in Rat Varicocele Model
Serefoglu EC, Kolbasi B, Bulbul MV, Karabulut S, Cakici C, Gundogdu Ozdemir RZ, Keskin I.
Abstract
Purpose
This study aimed to examine the therapeutic effects of injection of conditioned medium of adipose-derived mesenchymal stem cells (ADMSC-CM) in a surgically created varicocele model in comparison with varicocelectomy.
Materials and Methods
Twenty-eight male Wistar Albino rats were randomly divided into four groups: sham group, varicocele group, varicocelectomy group, and ADMSC-CM injection group. Sperm parameters were analyzed in samples taken from the epididymis after treatment. Malondialdehyde and superoxide dismutase (SOD) levels in blood samples were examined by biochemical analysis. The testicular tissues were stained with hematoxylin-eosin for histological examination (Johnsen’s Score). Additionally, Western Blot analyzes were performed to detect Claudin-11 levels, the functional protein of the blood-testis barrier, in testicular tissues.