Proteomics is the large-scale study of proteomes. The proteome is defined as all the proteins expressed by a cell, tissue or organism.
The proteome is inherently highly complex because proteins can undergo post-translational modifications (glycosylation, phosphorylation, acetylation, ubiquitination, and many other). Using proteomics technologies, it is possible to identify proteins in any tissue (brain, heart, liver, kidney, etc.) cell group/single cell or body fluid (blood, serum, cerebrospinal fluid (CSF), saliva, etc.).
The proteomic analyzes used in our laboratory are based on the analysis of the total protein mixture obtained from cell pellets, tissue (brain, liver, kidney), or body fluids (blood, serum, cerebrospinal fluid (CSF), saliva, etc.) in peptide structure by breaking down with trypsin enzyme without pre-separation. Protein identification process; It is described as bottom-up proteomics because it is in the direction of the protein, which is the whole of the identified peptides. By means of this method, protein identification is performed over partial sequences.
Omics technologies, which are rapidly renewing and improving themselves every day, make important contributions to science. Together with new technologies, a better understanding of biology and, accordingly, a more in-depth analysis of physiological mechanisms are provided.