Article / Archives of Physiology and Biochemistry
Humanin’s impact on pain markers and neuronal viability in diabetic neuropathy model
Muhammed Mirac Kelestemur, Ferah Bulut, Batuhan Bılgın, Munevver Gizem Hekım, Muhammed Adam, Sibel Ozcan, Mustafa Caglar Beker, Nalan Kaya Tektemur, Suat Tekin, Sinan Canpolat & Mete Ozcan
Abstract
Objective
This study investigates the impact of chronic humanin (HN) treatment on pain-related markers (NMDA, substance P, TRPV1, and IL-1β) in diabetic mice’s dorsal root ganglia (DRG). Additionally, we assess the effects of HN on cellular viability in DRG neurons.
Methods
In vivo experiments involved 15 days of HN administration (4 mg/kg) to diabetic mice (n = 10). Protein levels of NMDA, IL-1β, TRPV1, and substance P were measured in diabetic DRG. In vitro experiments explored HN’s impact on apoptosis and cellular viability, focusing on the JAK2/STAT3 pathway.
Results
Humanin significantly reduced the elevated expression of NMDA, IL-1β, TRPV1, and substance P induced by diabetes (p < .05). Furthermore, HN treatment increased cellular viability in DRG neurons through JAK2/STAT3 pathway activation (p < .05).
Conclusion
These findings highlight the significance of understanding mitochondrial function and pain markers, as well as apoptosis in diabetes. The study provides insights for managing the condition and its complications.