New Facility / Lightsheet – Fluorescence Microscopy

Light sheet fluorescence microscopy (LSFM) functions as a non-destructive microtome and microscope that uses a plane of light to optically section and view tissues with subcellular resolution. This method is well suited for deep imaging of transparent tissues or whole organisms. Tissues are exposed only to a thin plane of light resulting minimized photobleaching and phototoxicity compared to wide-field fluorescence, confocal, or multiphoton microscopy. LSFMs produce well-registered serial sections that are suitable for three-dimensional reconstruction of tissue structures.